Several pathogenic bacteria fall in the category of non-fastidious heterotrophic and therefore a complex media with peptone and beef extract makes the ideal media choice for their growth and culture. Blood Agar Media. Although similar to the idea of nutrient agar media, Blood agar media contains five to ten percent sheep or rabbit blood. Blood agar composition consists of Beef extract nitrogen source , Blood nitrogen, amino acids, and carbon source , Sodium chloride maintains osmotic balance , agar solidifying agent.
Blood Agar- Differential Media. Nutrient agar plates are commonly used for non-fastidious bacteria whereas blood agar plates are used by microbiologists for culturing fastidious pathogenic bacteria. These bacteria populations are selected by studying their hemolytic ability by which they destroy blood cells. To define an example, let us take Streptococcus pyogenes. These microorganisms can be grown on nutrient agar plates but if other bacteria are also growing, it gets difficult to distinguish this specific microbe.
In this scenario, blood agar media can be used. Chocolate Agar - Staphylococcus aureus Note: luxuriant growth with yellow pigmented colonies. Chocolate Agar - Neisseria gonorrhoeae Note: small colonies that appear transparent on close examination. Chocolate Agar - Escherichia coli Note: luxuriant growth of gray-white colonies. Thayer-Martin Agar. Thayer-Martin Agar - uninoculated. Type: Enriched and selective; contains antibiotics colistin kills gram-negative coliforms , vancomycin kills gram-positives , nystatin kills fungi.
Purpose: To select for fastidious organisms, such as N. Thayer-Martin Agar - Staphylococcus aureus Note: vancomycin in the medium inhibits the growth of staphylococci. Thayer-Martin Agar - Neisseria gonorrhoeae Note: luxuriant growth of this fastidious bacterium. Thayer-Martin Agar - Escherichia coli Note: colistin in the media inhibits the growth of enterics. MacConkey lactose Agar. MacConkey Agar - uninoculated. Purpose: Contains bile salts and crystal violet which selects for gram-negative enterics, differentiates lactose-fermenters from non-fermenters.
Can include sugars other than lactose for further differentiation for example, to differentiate enterohemorrhagic E.
Interpretation: Selects for non-fastidious gram-negatives; red colonies indicate fermentation of lactose, white indicates no fermentation of lactose. MacConkey Agar - Escherichia coli Note: Red colonies and red precipitate due to acid production as a result of lactose fermentation. Colorless colonies, medium is slightly yellow due to the increased pH resulting from bacterial digestion of peptone in the medium.
Pictures Four nutrient agar plates growing colonies of common Gram negative bacteria. Source: Wikipedia. Suspending 28 g of nutrient agar powder in 1 litre of distilled water.
That was for 1 plate For 6, it will be 4. MHA Reply. MHA… It can be used for any bacteria and contains starch which will regulate diffusion of other nutrients Reply. Can nutrient agar be used for a total bacterial count? What quantity of nutrient agar will be required to be suspended in ml of distilled water. Can Enterococcus faecalis grow on nutrient agar? We need 14g of nutrient agar powder to add to mls of distilled water. Could you please tell me what exactly we use nutrient agar for Thanks Reply.
Minly it is used for bacterial culture growth. Show the composition mode preparation and types of organism cultured media Reply.
Shall we use nutrient agar medium prepared without sodium chloride? Thanks Reply. Two types ke bacteria gram positive and gram negative Reply. For 1 L media we need 0.
To prepare 1 L media we need yeast extract 0. What about adding he chemical formula? What about nutrient broth? I dont know about that but you can add a broad spectrum antibiotic to avoid griwth of bacteria in your plates Reply.
What is Basal media???? What is Basal media Reply. It media that supports the growth of bacteria but do not need nutrients Reply. What is the difference between the blood agar plate and the nutrient agar plate? Category: medical health infectious diseases. Blood agar is almost identical to nutrient agar except that it contains five to ten percent sheep, rabbit, or horse blood.
Blood agar consists of: Beef extract, for nitrogen. Blood , for nitrogen, amino acids, and carbon. What bacteria can grow on nutrient agar? What is the purpose of agar? Why is nacl used in nutrient agar? Does E coli grow on nutrient agar? What color is E coli on nutrient agar?
Is MacConkey agar selective or differential? What are the components of nutrient agar? Is nutrient agar toxic? Is Agar Agar bad? Is agar agar powder healthy? Health Benefits. What are the types of agar? Types of agar plates[edit].
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